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The existence of contaminating sequences is bigger in low-biomass samples (such as from blood or perhaps the lung) than in large-biomass samples (including from faeces), suggesting that there is a essential tipping level wherever contaminating DNA will become dominant in sequence libraries.Check out the pH of the buffer periodically and assure opti
Initially, as opposed to the package's EDTA-containing elution buffer, ten mM Tris, pH7-8 is Ordinarily used for the final elution, specifically for samples which can be to become sequenced with PacBio chemistry, since even small quantities of EDTA can impede downstream procedures included in sequencing library preparing. Nuclease-free water with
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